The method of building a rabbit rectal VX2 carcinoma model by injecting the cell suspension of VX2 cells into the wall of the rectum guided by X-ray fluoroscopy is feasible. The advantages of the model are ease of establishment, short growth period, and high stability. The rectal VX2 carcinoma established in this rabbit model is similar to human rectal carcinoma in aspects of pathological representation, tumor development, and metastasis. It offers an ideal major animal model for the study of rectal carcinoma, and especially profits the study of the staging of rectal carcinoma in imaging.